Efficient control of group I intron ribozyme catalysis by DNA constraints.
نویسندگان
چکیده
Double-stranded DNA constraints enable efficient control of catalysis by a large multi-domain group I intron ribozyme.
منابع مشابه
Efficient control of group I intron ribozyme catalysis by DNA constraintsw
Rational attachment of double-stranded DNA as a structural constraint allows control over macromolecular conformation and function. Others have modulated the structure or enzymatic activity of a protein by changing the hybridization state of a DNA strand that is attached at both ends to the protein. We used DNA constraints to control the tertiary structure of the 160-nucleotide P4–P6 RNA domain...
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DiGIR1 is a group I-like ribozyme derived from the mobile twin ribozyme group I intron DiSSU1 in the nuclear ribosomal DNA of the myxomycete Didymium iridis. This ribozyme is responsible for intron RNA processing in vitro and in vivo at two internal sites close to the 5'-end of the intron endo-nuclease open reading frame and is a unique example of a group I ribozyme with an evolved biological f...
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The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors. Understanding how metal ions mediate catalysis requires elucidation of metal ion interactions with both the enzyme and the substrate(s). In the Tetrahymena group I intron, previo...
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After the discovery of RNase P (Chapter 9) and the Group I (Chapter 10) intron ribozymes, both of which are comparatively large and complex catalytic RNAs, the identification of the hammerhead ribozyme offered hope that the phenomenon of RNA catalysis might be best understood within the framework of a smaller, more tractable RNA that catalyzed a simple phosphodiester isomerization reaction. Ind...
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Covalent, crosslinking tethers have previously been attached to nucleic acids for control of structure and catalysis. Surprising results have been found, such as a study with the Tetrahymena group I intron ribozyme in which crosslinks that should substantially disrupt structure nevertheless did not suppress catalysis nearly as much as expected. As an alternative approach to control macromolecul...
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عنوان ژورنال:
- Chemical communications
دوره 7 شماره
صفحات -
تاریخ انتشار 2009